Abstract

SILVA-LEON, Giuliana; VELASQUEZ-HUAMAN, Zulema de los Ángeles  and  MAURTUA-TORRES, Dora Jesús. “In vitro” assessment of bacterial penetration resistance using two compaction techniques and two endodontic sealers against Enterococcus faecalis strain. Rev. Estomatol. Herediana [online]. 2015, vol.25, n.1, pp.18-26. ISSN 1019-4355.

Objectives: The purpose of the study was to assess “in vitro” bacterial penetration resistance using two different obturation techniques, vertical compaction and lateral compaction with two endodontic sealers, one based on polydimethylsiloxane and the other based on calcium hydroxide and epoxy resin against Enterococcus faecalis strain ATCC 29212. Methods: Eighty-four uniradicular teeth were randomly divided into 4 groups (n = 20) and two control groups (n = 4). Group I was obturated with lateral compaction technique and Sealer 26, group II with vertical compaction technique and Sealer 26, group III with lateral compaction technique and Roeko Seal and group IV with vertical compaction technique and Roeko Seal. Enterococcus faecalis was used as a bacterial marker and bacterial penetration was assessed monitoring the specimens every hour for the first 96 hours and after this every 12 hours over a period of 15 days. Results: Kruskal Wallis test (p = 0.696) was used to compare the four test groups and the Mann Whitney U test was used to compare pairs of groups. Nevertheless, no statistically significant differences were found comparing compaction techniques as endodontic sealers (Group I and II p=1.000, group III and IV p=0.296). No statistically significant differences were found when comparing endodontic sealers as compaction techniques (Group I and III p=0.328, group II and IV p=1.000). Although there was a maximum of 115 hours for group III and a minimum time of 18:21 hours for group I. Conclusions: No significant differences were found regarding bacterial penetration resistance between Roeko Seal and Sealer 26 in combination with lateral and vertical compaction techniques against a strain of Enterococcus faecalis.

Keywords : Sealer 26; Roeko Seal; Enterococcus faecalis.

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